WebMar 17, 2016 · Y187 yeast competent cells (Clontech) were then transformed with this construct according to the manual of Yeastmaker™ Yeast Transformation System 2 (Clontech). The expression of PfPP1 was checked by immunoblot using anti-GAL4BD antibody. This strain was used as a bait to screen protein interactions with the P. … WebAug 8, 2024 · A recent study has shown that zinc depletion induces autophagy by inhibiting TORC1 in yeast . Recently, many researchers have conducted studies on the effect of zinc on the autophagy process; however, the results have been inconsistent. ... (GAPDH) (internal control). The primers used were GAPDH-F:5′-GAG TCA ACG GAT TTG GTC …
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WebApr 13, 2024 · In the present study, the whole sequence of yeast pyruvate kinase (Cdc19p) was searched for condensate-forming regions. Four peptide fragments comprising 27–42 amino acids were found to form condensates. ... Unexpectedly, Adh1p conjugated with EGFP also formed foci when overexpressed under the GAPDH promoter in wild type … citizenship validation
Glyceraldehyde-3-phosphate dehydrogenase: a universal internal …
WebDec 1, 2011 · GAPDH as a substrate of yeast metacaspase during H 2 O 2-induced apoptosis. GAPDH fragmentation by metacaspase, during H 2 O 2-induced apoptosis, is … WebBackground: The use of reverse transcription-polymerase chain reaction (RT-PCR) to measure mRNA levels has led to the common use of β-actin and GAPDH housekeeping genes as denominators for comparison of samples. Expression of these genes is assumed to remain constant, so normalising for variations in processing and signal quantitation. … WebFeb 1, 2012 · G5537 Glyceraldehyde-3-phosphate Dehydrogenase from baker's yeast (S. cerevisiae) lyophilized powder, 70-140 units/mg protein Synonym (s): D-Glyceraldehyde 3-phosphate:NAD+ oxidoreductase (phosphorylating), GAPDH CAS Number: 9001-50-7 Enzyme Commission number: 1.2.1.12 ( BRENDA, IUBMB) EC Number: 232-609-4 MDL … dickies black puffer coat