H9c2 2-1 cells
WebCell information for H9c2(2-1). Find diseases associated with this biological target and compounds tested against it in bioassay experiments. WebMar 31, 2024 · The exposure of H9c2 cell lines to HG resulted in time- and concentration-dependent decreases in HIF-1α and HIF-2α expression together with an increase in prolyl hydroxylase-1,2 (PHD1 and PHD2) expression, the main regulators of …
H9c2 2-1 cells
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WebMar 16, 2024 · The expression and distribution of klotho in H9c2(2‑1) cells was observed by fluorogenic scanning, and the apoptotic rate was determined by Annexin … WebFeb 8, 2024 · The pcDNA3.1-MALAT1, MALAT1 siRNA (si-MALAT1) and their corresponding controls were purchased from Shanghai GenePharma Co., Ltd. H9C2 cells (2×105cells/ml) were transfected at 37°C for 24 h with Lipofectamine®2000 (Invitrogen; Thermo Fisher Scientific, Inc.) as previously described (20).
WebApr 4, 2024 · H9c2 cardiomyoblasts from rat embryonic cardiomyocytes were purchased from (ATCC, CRL-1446). Cells were cultured in complete DMEM (ATCC 30-2002), supplemented with 10% FBS (ATCC, 30-2024), and antibiotic penicillin/streptomycin (100 U/mL: 100 mg/mL) as described previously [ 14 ]. WebWhat is the recommended seeding density for H9C2 cells (specifically in a plastic tc t25 flask)? We have been seeding at the recommended 10x10^4/cm^2 (250,000 in t25) and …
WebPurchase high quality cryopreserved rat BDIX heart myoblast H9c2 cells from ECACC for myoblast differentiation, transfection, cardiomyocyte and muscle biology. US EN. … WebFeb 1, 2015 · 2.1. Cell cultures. H9c2 embryonic rat heart-derived (ventricular) cells (myoblasts) from ATCC were cultured in Dulbecco's modified Eagle's medium (DMEM), supplemented with 10% fetal bovine serum (FBS) under 95% air/5% CO 2, and subcultured when at 50–60% confluence. Notably, a higher degree of confluence was associated …
WebApr 4, 2024 · NC inhibited the phosphorylation of the mammalian target of rapamycin complex 1 (mTORC1) cascade and proinflammatory proteins in H9c2 cells. Top: …
WebIf use of this culture results in a scientific publication, it should be cited in the publication as: H9c2 (2-1) (ECACC 88092904) Skeletal muscle phenotype, no cardiac properties … lela shields artistWebCells were grown at 1.2 × 10 5 in 6-well plates. H9C2 cells were cultured in high glucose (HG, 33 mM) or normal glucose (NG, 5 mM) during 21 days, changing the media each … le lash lapeerWebH9c2 (2-1) CRL-1446 ™. H9c2 (2-1) is a subclone of the original clonal cell line derived from embryonic BD1X rat heart tissue that exhibits many of the properties of skeletal muscle. This cell line can be used in cardiovascular disease research. Product category. lelas fashionWebH9c2 cells were incubated with ET-1 in the presence or absence of KMUP-1 for 3 h. In order to measure ROS production induced by ET-1, cells were stained with 10 μM of DCFH-DA for 30 min at 37 °C, and immunofluorescence was visualized using a digital camera attached to an inverted microscope. Photographic images were taken of five random fields. lela\u0027s chickpea chipsWebSep 22, 2015 · For cell isolation, 1-2-day-old rat or mice were sacrificed, hearts were excised, atria were removed and ventricles were minced. ... H9C2 (2–1) (ATCC) rat cardiac muscle cells were cultured in DMEM … lela texas homes for saleWebThis paper aimed to analyze EVs released under normal and hypoxic conditions by H9c2 (rat), AC16 (human) and HL1 (mouse) cardiac cell lines. Small (sEVs), medium (mEVs) and large EVs (lEVs) were separated from a conditioned medium by a combination of gravity filtration, differential centrifugation and tangential flow filtration. lelather nova leather cleaner with microfiberWebMar 1, 2016 · And the population doubling time for H9C2 is 2.3 days if it grows well. So if I want it to achieve confluency at different day, I seed the cells according to split ratio as follows: 1: 2... lelant st ives holiday village