Hcrt promoter

Author: pihp

August undefined, 2024

WebAug 9, 2011 · Mice expressing ChR2 under the Hcrt promoter were stimulated with a blue laser diode (477 nm; 20 mW) through an optical fiber aimed at the lateral hypothalamus. Trains of 10 s (20 Hz, 15-ms light pulse) with 30- or 60-s intervals between the stimuli were used. ... Hcrt neurons are thought to participate in the homeostatic regulation of sleep by ... WebOct 17, 2007 · The 3,086-base-pair ( Eco RI– Sac I) mouse Hcrt promoter 19, 20 was used to replace the CaMKIIα promoter in the CaMKIIα::ChR2-mCherry lentivirus vector 25. The Hcrt::mCherry control viruses...

Sleep–wake regulation and hypocretin–melatonin interaction ... - PNAS

WebMar 15, 2015 · Loss of neurons that express the neuropeptide hypocretin (Hcrt) has been implicated in narcolepsy, a debilitating disorder characterized by excessive daytime … WebNov 22, 2024 · The lentiviral transduction of murine transcription factor ebf2 cDNA into 293 cells increased the gene expression driven by this minimal Hcrt‑gene promoter and an electrophoretic mobility shift... child support worksheet ohio

Neural substrates of awakening probed with optogenetic control …

WebTargeting the Hcrt system with optogenetics. To genetically target Hcrt neurons, we stereotaxically inject a virus carrying ChR2 driven by the Hcrt promoter into the lateral … WebHCRT neurotransmission promotes operant responding for both drug and non-drug rewards. ... and cannabis dependent subjects. Promoter methylation did not differ between … WebSep 24, 2024 · We show that the human HCRT gene promoter is methylation-sensitive in vitro, and is hypermethylated in the hypothalamus of patients selectively at a putative PAX5:ETS1 binding site within the proximal HCRT promoter. child support worksheet washington state

Mechanism for Hypocretin-mediated sleep-to-wake transitions.

WebSep 24, 2024 · We show that the human HCRT gene promoter is methylation-sensitive in vitro , and is hypermethylated in the hypothalamus of patients selectively at a putative … WebApr 18, 2011 · Presence of olf-1 (O/E3 binding site) like elements in the promoter region of prepro-orexin genes. Alignment of the upstream (283–300 bp) sequences of the prepro-orexin (prepro-hypocretin) locus (Hcrt) from four separate mammals shows two highly conserved olf-1-like (olf-1/ebf, bold face) elements. child support worksheets in ncWebFunctional Characterization of Zebrafish hcrt Promoter—We began the analysis of the hcrt promoter by studying the 1-kb functional regions … g pen ground material tank

"WebDec 12, 2024 · Hcrt-positive neurons are restricted to the perifornical hypothalamus (PFH) and lateral hypothalamic area (LHA) but have widespread projections to brain regions that differentially express HcrtR1 and HcrtR2, including dense projections to the noradrenergic areas of the locus coeruleus (LC), the nucleus accumbens, serotonergic areas of the … " - Hcrt promoter

Hcrt promoter

Modular organization of a hypocretin gene minimal promoter

WebJul 1, 2024 · The HCRT promoter was methylated in 25 of 41 (61%) cancer patients with depression and methylated in 7 of 23 (30%) of cancer patients without depression. … WebAug 10, 2007 · This important question can be investigated in a transgenic (TG) line of rats created to mimic the loss of HCRT neurons seen in human narcoleptics. In these rats, there is a gradual and selective loss of HCRT neurons as a result of a HCRT promoter-driven accumulation of the cytotoxic gene product poly-Q-ataxin-3 (Beuckmann et al., 2004).

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WebOct 6, 2006 · Functional analysis of the hcrt promoter through transient expression of EGFP. Zebrafish DNA fragments containing 1 or 2 kb of sequences upstream from the initiation codon of hcrt (2kb-zhcrt-EGFP and 1kb-zhcrt-EGFP, also called Δ2000–1000) are shown in gray. Vertical lines mark (250 bp) increments of the functional 1-kb promoter … WebSep 9, 2012 · We used the zebrafish 2-kilobasepair hcrt promoter to drive the expression of the nfsB-EGFP fusion protein specifically in HCRT neurons. This promoter was …

WebSep 25, 2012 · Current models of sleep/wake regulation posit that Hypocretin (Hcrt)-expressing neurons in the lateral hypothalamus promote and stabilize wakefulness by projecting to subcortical arousal centers. However, the critical downstream effectors of Hcrt neurons are unknown. WebConstructs—Zebrafish hcrt promoter constructs 2kb-zhcrt-EGFP ( 1941 to 62) and 1kb-zhcrt-EGFP ( 941 to 62, GenBankTM DQ831347) fragments were amplified by PCR of genomic DNA and ligated into the SalI-BamH1 sites of pEGFP-1. A 1040-bp T. nigroviridis hcrt promoter fragment (1–1040 of GenBankTM DQ831348) was amplified from BAC

WebSep 12, 2012 · We used the zebrafish 2-kilobasepair hcrt promoter to drive the expression of the nfsB-EGFP fusion protein specifically in HCRT neurons. This promoter was extensively used to characterize HCRT neuronal anatomy at high resolution, and ectopic expression of EGFP was never observed (Faraco et al., 2006; Appelbaum et al., 2009, … WebMar 15, 2015 · The boxed region is shown at higher magnification in the inset. (B) Schematic of the zebrafish 1 kb hcrt promoter, including putative Lhx9 binding sites A and B. (C) Sequence of a previously characterized mammalian Lhx9 binding site compared with sites A and B in the zebrafish hcrt promoter and in mutated constructs. Gray shading, …

WebNov 22, 2024 · The synthetic Hcrt promoter sequences were subcloned into the pNiFty3-Luc expression vector (InvivoGen, San Diego, CA, USA). This reporter plasmid encodes a coelenterazine-utilizing secreted luciferase and carries resistance to the antibiotic zeocin.

WebApr 11, 2024 · Methylation of the HCRT and GAL promoters was not associated with patients with HNSCC or the normal control group. A Pearson correlation revealed … child support worksheet utah orsWebDec 24, 2016 · The hypothalamic Hypocretin/Orexin (Hcrt) neurons secrete two Hcrt neuropeptides. These neurons and peptides play a major role in the regulation of feeding, sleep wake cycle, reward-seeking, addiction, and stress. Loss of Hcrt neurons causes the sleep disorder narcolepsy. gp enhanced access servicesWebThe boxed region is shown at higher magnification in the inset. (B) Schematic of the zebrafish 1 kb hcrt promoter, including putative Lhx9 binding sites A and B. (C) … child support wyoming lawsWebAbstract. Current models of sleep/wake regulation posit that Hypocretin (Hcrt)-expressing neurons in the lateral hypothalamus promote and stabilize wakefulness by projecting to … child support written agreementWebDec 22, 2009 · Hypocretin 1 and 2 (HCRT 1 and 2, also known as orexin A and B) are two neuropeptides originally isolated in rats, that are derived from a single gene precursor (Hcrt/Orx) (1, 2).HCRT preproprotein is exclusively expressed in neurons restricted to the lateral hypothalamus (LH) organized as a single compact cluster in each hemi-brain … child support york paWebDec 20, 2006 · Our zebrafish model of Hcrt overexpression indicates that the ancestral function of Hcrt is to promote locomotion and inhibit rest and will facilitate the discovery of neural circuits, genes, and drugs that regulate Hcrt function and sleep. hypocretin orexin sleep insomnia circadian rhythm zebrafish Introduction g pen herbal vaporizer with waxWebCurrent models of sleep/wake regulation posit that Hypocretin (Hcrt)-expressing neurons in the lateral hypothalamus promote and stabilize wakefulness by projecting to subcortical arousal centers. However, the critical downstream effectors of Hcrt neurons are unknown. child support zang blvd