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Lysotracker染色后固定

Web11 iul. 2013 · LysoTracker dyes do not specifically label lysosomes or autophagosomes within the cell, they allow the simultaneous measurement of an autophagy-related process and other live-cell functions, which are not possible with the standard LC3B antibody-labeling tech-nique. This method has the advantage of other live-cell LCB-GFP-tagged … Web25 sept. 2007 · In order to investigate the relationship of Lysotracker Red to the emergent green fluorescence, we compared the mean fluorescent signal obtained by excitation with a 488nm laser or a 594 nm laser ...

Lyso-Tracker Red(溶酶体红色荧光探针)_荧光染料_染料_染色试剂_ …

WebLysoTracker products provide fluorescence detection for live-cell staining of acidic environments and can be used for labeling and tracing acidic organelles such as lysosomes. For Research Use Only. Not for use in … Web16 apr. 2013 · Mitophagy is a specialized form of autophagy that removes damaged mitochondria, thereby maintaining efficient cellular metabolism and reducing cellular stress caused by aberrant oxidative bursts. Deficits in mitophagy underlie several diseases, and a substantial body of research has elucidated key steps in the pathways that lead to and … todaypost.com https://ccfiresprinkler.net

LysoTracker™ Deep Red

Web本品 LysoTracker® Green DND-26 为绿色荧光标记的溶酶体探针，具有 504/511 nm 的最大激发 / 发射波长。 本品以溶于无水 DMSO 的 1 mM 储存液形式提供。 产品性质 运输和保存方法 室温运输。 -20℃ 避光保存，避免反复冻融。 有效期 2 年。 注意事项 1 ） 为了您的安全和健康，请穿实验服并戴一次性手套操作。 2 ） 本产品仅作科研用途！ 使用方法 … WebLysoTracker™ Red DND-99 是一种红色荧光染料，可用于标记和追踪活细胞中的酸性细胞器。 LysoTracker™ 探针共有的特点包括： • 对酸性细胞器具有高度选择性 • 醛固定后保留良好 • 可有效标记纳摩尔浓度的活细胞 • 活细胞成像 • 激发/发射波长：577/590 nm 简单、高度特异性的一步法染色和酸性细胞器追踪 LysoTracker™ 探针由一种与弱碱结合的荧光 … Web2 sept. 2014 · LysoTracker is an acidotropic dye that stains cellular acidic compartments, including lysosomes and autolysosomes. LysoTracker has been used to detect … pension benefits act r.s.o. 1990 c. p.8

LysoTracker™ Green DND-26, special packaging - Thermo Fisher Scientific

Category:LysoTracker™ Red DND-99, special packaging

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Lysotracker染色后固定

溶酶体绿色荧光探针 溶酶体绿色荧光染料 LysoTracker Green …

WebCite. 14th Dec, 2024. Xiliang Du. Jilin University. Our results revealed that 4%paraformaldehyde fixed cell was OK to detect LysoTracker. The protocol was followed: cell was stained with ... WebMitoTracker 染料具有多种波长选择，可用于多色分析实验中的线粒体定位。 细胞固定后的染料保留情况：MitoTracker Deep Red 等染料固定后可充分保留，MitoTracker Red 则会固定后丢失。 请根据如下选择指南查找适合您实验的染料。 选择 MitoTracker 标记探针 固定细胞线粒体染色的偶联物 使用抗线粒体蛋白的荧光抗体偶联物，可标记固定细胞中的线粒体。 …

Lysotracker染色后固定

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Web2 sept. 2014 · LysoTracker is an acidotropic dye that stains cellular acidic compartments, including lysosomes and autolysosomes. LysoTracker has been used to detect autophagy-associated lysosomal activity in Drosophila tissues including the fat body, midgut, salivary gland and ovary, as well as in Drosophila cell culture. A low level of LysoTracker … WebDescription. LysoTracker Red DND-99 is a cell-permeable red fluorescent dye that stains acidic compartments within a cell, such as lysosomes. Red DND-99 has an excitation and emission maximum of 577/590 nm and can be efficiently excited using a TRITC filter. LysoTracker probes are available in a variety of fluorescent colors.

WebAbstract. The flow cytometric use of LysoTracker dyes was employed to investigate the autophagic process and to compare this with the upregulation of autophagy marker, the … WebThe flow cytometric use of LysoTracker dyes was employed to investigate the autophagic process and to compare this with the upregulation of autophagy marker, the microtubule-associated protein LC3B. Although the mechanism of action of LysoTracker dyes is not fully understood, they have been used in microscopy to image acidic spherical ...

WebLysoTrackerマーカーは、弱塩基性アミンに結合したフルオロフォアで構成されています。LysoTracker保持のメカニズムは比較的不明ですが、オルガネラの膜に保持された状 … Web11 oct. 2012 · Use of LysoTracker to detect programmed cell death in embryos and differentiating embryonic stem cells. Programmed cell death (PCD) occurs in adults to …

WebLysoTracker ® 系列探针是对活细胞中的酸性区室进行选择性染色的一类荧光染料，该类探针具有几大重要的特点，1）选择性标记酸性细胞器；2）纳摩尔级（nM）浓度即可有效 …

WebLysoTracker® Green DND-26 是一种可渗透细胞的绿色染料，能对活细胞中的酸性区室（溶酶体）进行染色。 限制使用 除非 CST 的合法授书代表以书面形式书行明确同意，否书 … pension benefit information companyWeb14 mai 2024 · Live-cell imaging of Lysotracker-stained cells showed that loss of fluorescence occurred within a minute after LLOMe exposure (Supplementary Video 1), demonstrating an instant damage to the ... pension benefit accountWeb可以的。 注意几点: 1.lysotracker和Hochest都可以染活细胞，直接加进培养基里就行，染完用PBS洗掉，即可拍照。 2.一般lysotracker是红色或者绿色通道的荧光，Hochest是蓝色的，可以兼容。 3.不同细胞着色程度有差异，建议调整下染料浓度和染色时间，第一次染时可以在荧光显微镜下每隔10分钟观察一下，或者请教有经验的师兄师姐。 编辑于 2024-12 … pension benefit lawyersWeb如果经Lyso-Tracker Red染色后的细胞需要进行固定操作，可以尝试3%的戊二醛 (glutaraldehyde)。 按照1:20000的比例稀释，可以配制1000ml Lyso-Tracker Red工作液。 包装清单： 保存条件： -20℃避光保存，半年有效。 注意事项： Lyso-Tracker Red (1mM)在4℃、冰浴等较低温度情况下会凝固而粘在离心管管底、管壁或管盖内，可以20-25℃水 … pension benefits act ontario regulation 909WebFor confocal, I use lysotracker setting, and laser is 2%, with pin hole slightly larger than 1 airy unit (~1.3um). Digital gain isn't high, and no subtraction since there is no noise other than ... pension benefit obligation accountingWebLysoTracker 是细胞渗透性溶酶体标记物，靶向并示踪活细胞中的酸性细胞器。 LysoTracker 标记物由与弱碱性胺结合的荧光基团组成。 虽然 LysoTracker 的滞留机制尚未完全明 … pension benefits act skpension benefits act manitoba