Polysome profiling and cancer

Author: rkjh

August undefined, 2024

WebOct 29, 2024 · Beatrice Hunter Cancer Research Institute, Halifax, Nova Scotia, Canada. Corresponding author: [email protected] Search for more papers by this author. ... WebMay 17, 2014 · This work shows that analysis of partial variance, which produces estimates of translational activity that are independent of cytosolic mRNA levels, is a superior alternative and is applicable to datasets obtained from both the commonly used polysome microarray method and the sequencing-based ribosome profiling method. Expand

CDK13 phosphorylates the translation machinery and promotes …

WebThe structure of proline prevents it from adopting an optimal position for rapid protein synthesis. Poly-proline-tract (PPT) associated ribosomal stalling is resolved by highly conserved eIF5A, the only protein to contain the amino acid hypusine. We WebFeb 19, 2024 · Polysome profiling is a widely used method to monitor the translation status of mRNAs. Although it is theoretically a simple technique, it is labor intensive. Repetitive polysome fractionation rapidly generates a large number of samples to be handled in the downstream processes of protein elimination, RNA extraction and quantification. Here, we … flappy bird board game

Deregulation of ribosomal protein expression and translation

WebMy specialist areas are Ion Torrent PGM sequencing, using Ampliseq and RNA-seq in particular. This work goes towards cancer mutation analysis and target identification. Other skills include polysome profiling, for gene expression and miRNA studies. My key responsibilities are to manage the… Show more WebHere, we describe the polysome profiling technique and RNA as well as protein isolation procedures from sucrose fractions ... Girault I et al (2014) eIF4F is a nexus of resistance … WebGlioblastoma (GBM) is one of the most aggressive cancers, with median survival of less than 2 years. Despite of considerable advance in molecular classification of GBMs, no improvements in therapy have been described. The scenario is further complicated by tumor heterogeneity and the relationship among genetic, transcriptional and functional findings. … flappy bird brother kills brother

Hypoxia Induces Autophagy through Translational Up-Regulation …

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WebOct 29, 2024 · Beatrice Hunter Cancer Research Institute, Halifax, Nova Scotia, Canada. Corresponding author: [email protected] Search for more papers by this author. ... Polysome profiling analysis utilizes ultracentrifugation to isolate complexes of mRNAs in the process of translation and corresponding proteins on the basis of density. WebMay 17, 2014 · An example of applying the polysome fractionation method to investigate the role of mTOR signaling in mediating the effects of insulin on mRNA translation is presented. To this end, MCF7 human breast cancer cells were maintained in low serum and then stimulated with insulin alone or in combination with the active-site mTOR inhibitor Torin1. flappy bird browserWebFeb 6, 2024 · Polysome profiling also identified a significant increase in the global polysome-to-monosome ratio in RPL15-CTCs (fig. S6, B and C), indicating a global ... Raw data from RNA-seq of primary human breast cancer CTCs and ribosome profiling of RPL15-CTCs and microarray data from polysome profiling of TGF-β–treated MCF10A cells ... can snakes swallow people whole

"WebContributed to four years of grant renewal for Dr. Blackshear’s clinical research laboratory by NIH with establishment of polysome profiling methodologies for mouse fibroblast cells and ... " - Polysome profiling and cancer

Polysome profiling and cancer

Polysome Fractionation and Analysis of Mammalian …

WebA complex interplay between mRNA translation and cellular respiration has been recently unveiled, but its regulation in humans is poorly characterized in either health or disease. Cancer cells radically reshape both biosynthetic and bioenergetic Web(A) Heat map of distribution in the polysome gradient based on 3′-seq of 199 of the short 3′ UTR mRNA isoforms that predominantly comigrated with fractions lighter than the 80S monosome at the 48-h PHS time point from Figure 4 F. (B) Heat map of distribution in the polysome fractions from 72-h PHS testes of the same 199 short 3′ UTR mRNA isoforms …

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WebExploring the field of epitranscriptomics and its implications in human cancer cell lines to identify potential drug targets. • Generated NSUN2 KO human cancer cell lines using CRISPR- Cas9. • Performed downstream analysis of NSUN2 KO cells (polysome and ribosome profiling and sequencing) and I am currently working on the functional validation. WebApr 11, 2024 · The disease is the leading cause of cancer death, according to the American Cancer Society, which estimates that this year there will be more than 238,000 new cases of lung cancer and more than ...

WebApr 14, 2016 · In this study, we used polysome profiling coupled to whole human genome expression array to identify candidate genes whose translation is regulated by hypoxia in … WebMay 12, 2016 · Changes in polysome-bound mRNA (translatome) are correlated closely with changes in the proteome in cells. Therefore, to better understand the processes mediating the response of glioblastoma to ionizing radiation (IR), we used polysome profiling to define the IR-induced translatomes of a set of human glioblastoma stem-like cell (GSC) lines.

WebMar 7, 2024 · Polysome profiling analysis shows that MYC oncoprotein synthesis strictly depends on CDK13-regulated translation in colorectal cancer (CRC), and CDK13 is … WebOct 27, 2024 · Unlike other types of breast cancer that can be effectively treated by targeted therapies, ... G Polysome profiling of MDA-MB231 cells with or without ADAR1 knockdown.

WebIntroduction. Studies into the genomics, epigenetics, and transcriptomes of cancer have yielded important insights into its pathogenesis. However, proteomic studies revealed that the transcriptome does not always predict the proteome (de Sousa Abreu et al, 2009).This disconnect is due, in part, to post-transcriptional regulation, e.g., splicing, nuclear RNA …

WebROYGBV colors (with dashed lines as boundaries) indicate the polysome fractions that were combined before 3′-seq. (C,E) 3′-seq from polysome profiling from 24-h PHS testes, plotting relative level of the long 3′ UTR isoform detected across the different polysome fractions (in each column) for genes that undergo the stage-specific 3′ UTR shortening due to APA (in … flappy bird boruWebMiR-210 is induced by hypoxia and plays different roles in the development of certain cancers. However, little is known about its role in pancreatic cancer (PC). This study aimed to explore the induction and modulation of PC by miR-210 and its potential molecular targets. Methods. can snakes tie themselves up in a knotWeb2 Beatrice Hunter Cancer Research Institute, Halifax, Nova Scotia, Canada. PMID: 30371019 ... Polysome profiling analysis utilizes ultracentrifugation to isolate complexes of mRNAs … can snakes slither backwardsWebFeb 2, 2024 · The development and utilization of the polysome profiling technique for oocytes and zygotes have made the identification of actively translating mRNAs during MZT possible . Recently, an oocyte-specific (Zp3-Cre) RiboTag strategy has been used to characterize the dynamics of mRNA translation during oocyte maturation, which revealed … can snakes tie themselves in knots and dieWebFeb 5, 2024 · The impact of factors that regulate selective mRNA translation is a critical question in RNA biology. Polyribosome (polysome) fractionation analysis is a powerful … can snakes turn into humanWebJan 1, 2024 · Polysome-profiling of bio-banked breast cancer tissues using optimized non-linear gradients and smartSeq2 To comprehensively evaluate the non-linear sucrose … flappy bird bossWebDec 22, 2024 · To validate whether loss of the m 6 A1832 modification could lead to alterations of ribosome function and polysome profile, we performed sucrose gradient … flappy bird browser game